Q1: A white precipitate occurred after thawing frozen samples stored in ViShield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
Q2: Do I need to homogenize the sample in ViShield prior to storage?
No, tissues do not need to be homogenized.
Q3: How to collect solid tissues in ViShield?
Transfer tissues in to ViShield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
Q4: Can I FACS sort directly into ViShield?
Yes, sort directly into ViShield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of ViShield).
Q5: How long can samples be stored frozen in ViShield?
DNA & RNA (<-20°C): Indefinitely
Q6: How long can samples be stored samples in ViShield at ambient temperature?
RNA > 1 month
DNA > 2 years
Q7: What sample types is ViShield suitable for?
ViShield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q8: Can ViShield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in ViShield. Prior to downstream applications, clean-up the sample via the appropriate Vitaimed clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).